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Figure 8


Fig. 8. Cdk5 phosphorylates paxillin and forms a complex with paxillin after OL differentiation. (A) Primary OLs were immunostained with antibodies against Cdk5 (red) and paxillin (green) before or 3 days after induction of differentiation. Panels a-f are magnifications of the boxed areas as indicated. (B) Three days after induction of differentiation, immunoprecipitates of endogenous Cdk5 and paxillin in the lysates of OLs were immunoblotted with their respective antibodies. The total proteins levels of Cdk5 and paxillin are also shown. (C) Immunoprecipitates of endogenous paxillin from the lysates of OLs treated with or without 10 µM roscovitine immunoblotted with antibody recognizing phosphorylated Cdk substrate before or 3 days after induction of differentiation. Total protein levels of paxillin and β-actin are also shown. (D) Quantification of the intensity of the bands shown in the immunoblots. Data were evaluated using Student's t-test (*P<0.01). (E) Before or 3 days after induction of differentiation, immunoprecipitates of endogenous FAK in lysates of OLs treated with or without 10 µM roscovitine were immunoblotted with antibody against paxillin. Total protein levels of paxillin and FAK are also shown.





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