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Figure 3


Fig. 3. NCAM is sufficient for the regulation of the cellular response to FGF. (A) L cells stably transfected with empty vector (L-mock), full-length NCAM or {Delta}FN2 were stimulated for 10 minutes with FGF2, followed by cell lysis and immunoblotting for phospho-ERK1/2 and then for total ERK1/2. (B) L cells stably transfected with empty vector, full-length NCAM or {Delta}FN2 were left untreated or were treated with FGF2 for the indicated time periods. Cell proliferation was assessed as described in the Materials and Methods. The growth curves of untreated L-NCAM and L-{Delta}FN2 cells, which were very similar to that of untreated L-mock cells (see also supplementary material Fig. S4A), have been omitted for simplicity. *P<0.005, relative to untreated L-mock cells.





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