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Fig. 3. Root growth defects in clasp mutants. (A) Ten-day-old wild-type Columbia (Col) and clasp seedlings. Right panel shows a magnified image of the clasp seedlings. Note increased formation of lateral roots. (B) CYCB1;1:GUS expression in wild-type and clasp root tips. Brackets indicate a zone of active cell division as highlighted by CYCB1;1:GUS expression. Note the smaller number of stained cells and the reduction of the zone in which GUS-stained cells are observed. (C) Comparison of the cell elongation zone in the wild type and the clasp mutant root. Brackets span the position of the first elongated cell in the epidermis and the first cell initiating root hair formation. (D) Quantification of cell number in the root elongation zones of wild-type and mutant seedlings. n=12 roots for clasp seedlings and 17 roots for the wild type. Error bars indicate s.d. The differences between means for both epidermal and cortical cells is significant by Student's t-test, P<0.001. (E) Quantification of mature epidermal cell length in wild-type and clasp roots. n=28 cells for wild type and n=51 cells for clasp mutants. The difference in means is significant by Student's t-test, P<0.001. Scale bars: 100 µm (B), 150 µm (C).

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