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Figure 9


Fig. 9. YFP:AtCLASP is enriched at the MT plus end. (A) Time-series images acquired at the cortex of a hypocotyl cell expressing CFP:EB1 and YFP:AtCLASP proteins, both expressed from the 35S promoter. Both proteins show localization to the growing ends of cortical MTs (arrowheads). (B) The relative position of EB1 and CLASP was determined by plotting the normalized fluorescent intensity (NFI) along the MT ends in a 5-pixel-wide lane. Normalized fluorescent intensity (NFI) of CFP:EB1 is in red and YFP:AtCLASP in green. The graph shows the mean values of 12 measurements. The measured difference in peak maxima position is significant by Student's t-test, P<0.001. A magnified image of representative MT end is shown below. Scale bar: 2 µm.





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