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Figure 7


Fig. 7. Reggie-1 colocalizes with the late endosomal marker LAMP-3 and with the EGF receptor. HeLa cells transfected with R1-EGFP were starved for 16 hours and then stimulated with EGF (100 ng/ml) for 15 minutes. Staining for LAMP-3 revealed a strong colocalization with reggie-1 (A). A partial colocalization of reggie-1 with EGF receptor could also be detected (B). By contrast, no significant colocalization could be observed between R1-EGFP and the early endosomal marker EEA1 (C) or transferrin receptor (D), which serves as a marker for recycling endosomes. Images in the right-hand column show magnifications of the colocalization.





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