|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Bacterial sphingomyelinase overexpression inhibits AVP-induced myogenic response. To induce the synthesis of endogenous ceramide, L6 cells were transfected with a vector allowing the expression of ER-targeted, GFP-tagged sphingomyelinase. Mock-transfection with a vector lacking the sphingomyelinase sequence was also performed. The transfected cells were cultured for 24 hours in 1% BSA medium in the absence (control) or presence of 10-7 M AVP, and nuclear myogenin immunofluorescence was assessed with a primary anti-myogenin monoclonal antibody and a secondary anti-mouse IgG-rhodamine conjugate. (A) Mock-transfected cells + AVP. (B) Sphingomyelinase-transfected cells + AVP. Bar, 20 µm. The graph shows the means ± s.e.m. of the percentages of myogenin-positive cells as calculated in the sole subsets of green fluorescent cells, in each condition (n=10 fields with 
10-15 cells per field).
|
