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Figure 3


Fig. 3. Immunolabeling of BAX (red) and GCNA1 (green) in Bax+/+ ovaries. Bar, 100 µm. (A) 12.5 d.p.c. faint but distinct BAX labeling is seen in the cytoplasm of both germ and somatic cells in the entire ovary. No GCNA1 labeling in this section, although some cells show characteristics of germ cells, such as large and round nuclei (arrowhead). (B) 14.5 d.p.c. More intense BAX labeling in both germ cells, identified with GCNA1 labeling (arrowheads), and somatic cells, compared with 12.5 d.p.c. (C) 18.5 d.p.c. Intense BAX labeling in both germ (arrowheads) and somatic cells. (D) 21.5 d.p.c. Intense BAX labeling in the cytoplasm of oocytes (arrowheads), particularly of large oocytes in the central region; BAX labeling in somatic cells has diminished. GCNA1 labeling is only in the germ cells in the peripheral region.





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