spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. The increase in the association of SgIF4G and eIF4E following fertilization correlates with the dissociation of eIF4E and 4E-BP, and occurs after modifications of SgIF4G protein. (A) SgIF4G associates with eIF4E when 4E-BP dissociates from eIF4E. m7GTP-column affinity-purified egg proteins extracted prior to or at the indicated times after fertilization were resolved by 15% SDS-PAGE and subjected to western blotting using anti-4E-BP2 (bottom), anti-eIF4E (middle) and anti-SgIF4G (top) antibodies. (B) SgIF4G modifications occur rapidly following fertilization. Supernatants obtained from low-speed centrifugation of egg homogenates prepared prior to or at the indicated times following fertilization, were resolved by 7.5% SDS-PAGE and subjected to western blotting using anti-SgIF4G (top). Anti-actin was used as loading control (bottom).





Right arrow Return to article