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Figure 6


Fig. 6. Microinjection of m7GDP inhibits the first mitotic division of sea urchin embryos. m7GDP was introduced by microinjection at a final intracellular concentration of 1 mM into unfertilized eggs. The control corresponds to non-injected eggs and buffer corresponds to eggs injected with the microinjection buffer. Cleavage was scored by observation under a light microscope at 150 minutes post-fertilization. An average of 60 unfertilized eggs were injected for each compound in each experiment, and error bars represent the standard deviation (± s.d.) of three independent experiments. Significance was assessed using Fisher's F-test and Student's t-test. *P<0.005, significant difference between eggs microinjected with m7GDP, microinjected with buffer and non-injected eggs.





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