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Fig. 5. Spindle-related phenotypes resulting from substitutions cancelling S36 and S91 putative CDK sites. (A) Distribution of MT-based structures in cell populations of the indicated strains expressing a GFP-Tub1p fusion. At least 500 cells were counted in duplicate experiments. Error bars indicate s.e.m. (B) Cell distribution by spindle length at onset of anaphase B. At least 200 digital images of synchronised cells of the indicated strains expressing GFP-Tub1p were used for measuring spindle length (see Materials and Methods). In wild-type cells the majority of spindles are either shorter than 2 µm or already longer than 3.5 µm as they initiated the fast phase of spindle elongation. Accumulation of spindles in the range of 2-3 µm, in particular, in the spc11091A mutant indicated a slow point or failure to engage in the fast phase of spindle elongation.
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