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Figure 6


Fig. 6. Spindle dynamics in wild-type or spc11091A mutant cells at onset of spindle elongation. (A,B) Selected frames from time-lapse series for representative spindle dynamics in wild-type cells expressing GFP-Tub1p. The fast phase of spindle elongation started in a single transition (6-8.5 minutes in A; 2-6 minutes in B). (C,D) Selected frames from time-lapse series for representative spindle dynamics in spc11091A mutant cells expressing GFP-Tub1p. Spindle length increased slowly (4.5-11 minutes in C; 0-6.5 minutes in D) before spindle elongation proceeded at a rate characteristic of the fast phase of wild-type cells. (E) Plot of kinetics of spindle elongation in a wild-type cell vs spc11091A mutant cell. Spindle measurement in digital images from live cell recordings was carried out as previously described (Segal et al., 2000b). Bars, 2 µm.





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