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Figure 7


Fig. 7. Effect of a mad2{Delta} mutation on spindle dynamics of spc11091A mutants. (A) Cell distribution by spindle length at onset of anaphase B in the indicated strains expressing GFP-Tub1p. Deletion of MAD2 abolished the accumulation of cells exhibiting spindle lengths in the range of 2-3.2 µm. (B) Distribution of MT-based structures in cell populations of the indicated strains expressing a GFP-Tub1p fusion. A MAD2 deletion eliminated the metaphase delay of spc11091A cells. Error bars indicate s.e.m. (C) Plot of kinetics of spindle elongation in spc11091A vs spc11091A mad2{Delta}. The mad2{Delta} mutation allowed an spc11091A cell to initiate the fast phase of spindle elongation in a single transition.





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