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Figure 7


Fig. 7. Filamin cleavage controls the localization of p190RhoGAP. (A) Raft-localization and cleavage of filamin in HMVE cells that were cultured in suspension (Susp) or plated on fibronectin in the absence (Spread) or presence of cytochalasin D (CytoD), latrunculinB (LatB), or ALLN. (B) Raft-localization of p190RhoGAP in control versus ALLN-treated filamin knockdown cells. Quantitative results were normalized to spread control cells (*P<0.01). (C) Filamin protein in total cell lysate from M2 cells transfected with intact GFP-filamin (GFP-fil) or a GFP-filamin-{Delta} (GFP-fil-{Delta}) construct that lacks the calpain cleavage site. (D) Raft-localization of p190RhoGAP in total cell population of M2 cells transfected with GFP-fil or GFP-fil-{Delta}. (E) Levels of active RhoA in total cell population of M2 cells transfected with GFP-fil or GFP-fil-{Delta} (*P<0.02).





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