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Figure 2


Fig. 2. CDβgeo cells can self-renew in suspension culture and express putative stem cell markers. (a) Bright-field image. Approximately 1 in 600-1000 CDβgeo cells forms a mammosphere. Bar, 100 µm. (b-e) CDβgeo mammospheres were cytospun onto glass slides, and immunostained for CD49f (b, red. Bar, 20 µm), TIE-2 (c, red. Bar, 20 µm), K14 (d, red. Bar, 50 µm), and keratin 6 (e, green. Bar, 50 µm). All nuclei were counterstained with DAPI (blue).





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