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Figure 3


Fig. 3. Stabilized β-catenin expression enriches for stem/progenitor cells by increasing the number of mammospheres. CDβgeo cells transduced with GFP (control), β-catenin, or β-engrailed were FACS sorted into Sca1+ and Sca1- populations and grown in suspension at a density of 20,000 cells per well for 14 days. The mammospheres were passaged once after 7 days, and the secondary mammospheres were counted using a Leica dissecting microscope. GFP control Sca1+ vs Sca1- *P<0.02; GFP Sca1+ vs β-catenin Sca1+, **P<0.008. The efficiency of mammosphere formation is calculated as the number of mammospheres per the number of plated cells. Data were collected from three individual experiments performed in triplicate.





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