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Figure 4


Fig. 4. Quantitative analysis of TbCMF cell motility. (A) Cell traces of uninduced and induced (30 hpi) TbCMF 46 cells. Analysis was done as described in Materials and Methods. Running cells (R) were classified as having sustained propulsive movement for at least 5 seconds. Tumbling cells (T) had visible movement, but did not sustain a run for 5 seconds or more. Immotile cells (I) were classified as cells where movement was visible at the cellular level, but the cell did not move from the point of origin. (B) Graphical representation of motility differences between uninduced and induced mutant cells. Class 2, TbCMF 2 and TbCMF 3; Class 3, TbCMF 46 and TbCMF 76b; Class 4, TbCMF 5 and TbCMF 9. n=49-53 cells; 30-second interval. Blue, immotile cells; red, tumbler cells; yellow, runner cells. Bars, 50 µm.





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