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Fig. 4. Rho1 signals through Diaphanous to activate Rac1. (A-D) The hemocyte actin cytoskeleton was visualized using Alexa Fluor 546-phalloidin (red) and the nucleus was stained with DAPI (blue). (A) Hml-Gal4, (B) dia^k07135/UAS-Rok^catkg;UAS-Rho1^V14/Hml-Gal4, (C) Hml-Gal4/Dia^CA,(D) Hml-Gal4/Dia^CA [anti-Diaphanous (red), Alexa Fluor 488-phalloidin (green)]. (E) F-actin expression levels. Hml-Gal4 was crossed with different UAS constructs and hemocytes were bled from wandering third instar larvae. The hemocytes were stained with Alexa Fluor 546-phalloidin. Imagetrak was used to measure fluorescence intensity of at least 100 hemocytes from three different larvae. An asterisk indicates a significant difference (Student's t-test, P<0.01). (F) Hemocyte cell counts. Hemocytes were counted from at least 15 individual larvae. An asterisk indicates a significant difference (Student's t-test, P<0.01) compared with the Hml-Gal4 strain.

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