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Fig. 5. Dominant negative Cdc42 can block Diaphanous-induced filopodia. (A) Plasmatocytes recovered from Hml-Gal4;DiaCA/Rac1N17 or Hml-Gal4/Cdc42N17;DiaCA larvae and stained with anti-Diaphanous (red) and Alexa Fluor 488-phalloidin (green). In the merged images, overlap of expression appears yellow. (B) Cell counts of hemocytes expressing multiple filopodia. Hemocytes were counted from at least five individual larvae. An asterisk indicates a significant difference (t-test, P<0.01) compared with the Hml-Gal4 strain.