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Fig. 3. xLBR domain requirement for NE localization and aggregation production in both HeLa and XTC cells. (A) GFP-fused LBR or its deletion mutants were overexpressed in HeLa or XTC cells. The cells were examined under a fluorescence microscope. Note that GFP-xLBR^1-210 was concentrated in nucleoplasm, whereas the GFP-LBR^90-210 was distributed throughout the whole cell, indicating that a nuclear localization signal is present within amino acids 1-90. GFP-LBR^211-621 was located on the nuclear rim and forms aggregates although less than those of full length xLBR, whereas GFP-LBR^309-621 was located in the whole cell body, suggesting that the first transmembrane segment within amino acids 211 to 309 is necessary for locating the protein to the NE and formation of the aggregates. Bar, 10 µm. (B) A schematic diagram of the domain structure of LBR.

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