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Figure 2


Fig. 2. Chelatable, redox-active Fe accumulation in wheat leaves after Bgt attack. (A) Wheat epidermis 24 hpi with Bgt stained for Fe3+ after pretreatment with the Fe3+ chelator DFO. (B) In situ calcein staining of Fe3+ in wheat leaves 24 hpi with Bgt before (left panel) and after (right panel) treatment with the Fe chelator DFO. (C) Wide-range X-band EPR spectrometry of wheat leaves 24 hpi with (blue solid line) or without (black broken line) Bgt inoculation. Insets are the enlarged high-resolution scanning for high-spin and low-spin Fe3+, respectively. The g values of high-spin Fe3+ are indicated. Spectral intensities were normalized relative to sample amount. Two independent experiments showed similar results. agt, appressorial germ tube; c, conidium; ha, halo; gc, guard cell; N, epidermal nucleus; pa, papilla. Scale bar, 20 µm.





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