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Figure 4


Fig. 4. Fe overload causes apoplastic H2O2 production and induces Fe homeostasis- and defense-related gene expression. (A) ICPMS analysis of Fe3+-EDTA uptake in wheat leaves. Values represent mean ± s.d. (n=3). (B) DAB staining for H2O2 in wheat leaves 12 hours after treatment with either Fe3+-EDTA (middle and right panels) or EDTA (Mock, left panel). (C) Northern blot analysis of time-dependent induction of Fe homeostasis- and defense-related genes following loading with 500 µM EDTA (Mock) or 500 µM Fe3+-EDTA at the time points indicated. Even loading of total RNA was monitored by ethidium bromide staining of rRNA. (D) Northern blot analysis of Fe3+-EDTA concentration-dependent gene expression. (E) Northern blot analysis of Fe3+-dependent and H2O2-dependent gene expression.





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