spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. H2O2 promotes cytosolic Fe depletion and Fe efflux. (A,B) Confocal imaging of Fe depletion in wheat suspension cells, based on calcein-AM fluorescence. Images (lower panels) depict the fluorescence at representative time points. Scale bar, 20 µm. Time course of calcein-AM uptake (A); H2O2-mediated cytosolic Fe depletion (B). (C,D) H2O2-induced Fe efflux in wheat suspension cells. Bar values represent mean ± s.d. (n=3). Cells were treated with glucose/glucose oxidase (G/GO) (5 mM/5 U ml–1) or H2O2 (10 mM), with or without catalase (Cat) (10 U ml–1) or vanadate (NaVO3) (100 µM), respectively (C); cells treated with 10 mM H2O2 and 20 or 200 µM cycloheximide (CHX) (D).





Right arrow Return to article