JCS03375 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. 3D image of a representative wild-type PC12 cell stained with Syt I-specific (red channel) and Syt IX-specific (green channel) antibodies showing colocalization (yellow) to punctate structures. 3D image was reconstructed from a deconvolved confocal Z-series through the cell at 250 nm step size. The Movie corresponds to still images of Fig. 1A. A better quality file can be found at http://www.biochem.wisc.edu/martin/lynch/fig5video1.html

• Movie 2 -

  Movie 2. Two representative wild-type PC12 cells that express ANF-EGFP and rest in basal media. Images were acquired in TIRF at 0.25-second intervals and displayed at a rate of 30 frames per second. Bar, 10 μm. A better quality file can be found at http://www.biochem.wisc.edu/martin/lynch/fig5video1.html

• Movie 3 -

  Movie 3. Two representative Syt I/IX-null PC12 cells that express ANF-EGFP and rest in basal media. Images were acquired in TIRF at 0.25-second intervals and displayed at a rate of 30 frames per second. Bar, 10 μm. A better quality file can be found at http://www.biochem.wisc.edu/martin/lynch/fig5video2.html

• Movie 4 -

  Movie 4. Representative wild-type PC12 cell expressing ANF-EGFP, immediately after stimulation with depolarizing high K⁺ media. Fusion events appear as flashes of fluorescence. Images were acquired in TIRF at 0.25-second intervals and displayed at a rate of 30 frames per second. Bar, 10 μm. A better quality file can be found at http://www.biochem.wisc.edu/martin/lynch/fig5video3.html

• Movie 5 -

  Movie 5. Representative Syt I/IX-null PC12 cell expressing ANF-EGFP, immediately after stimulation with depolarizing high K⁺ media. Images were acquired in TIRF at 0.25-second intervals and displayed at a rate of 30 frames per second. Bar, 10 μm. A better quality file can be found at http://www.biochem.wisc.edu/martin/lynch/fig5video4.html