JCS03357 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Confocal fluorescence microscopy experiments showing the subcellular distribution of endogenous PRMT1 in MCF-7 cells visualized using the polyclonal anti-PRMT1 from Upstate Biotechnology. The corresponding phase-contrast micrograph is shown in the right-hand panel.

• Supplemental Figure 2 -

  Fig. S2. Image processing of hCAF1 and PRMT1 colocalization on a section of nucleus performed using LSM software (Zeiss). Analysis shows a global colocalization percentage of 51.3% and two peaks at 100%.

• Supplemental Figure 3 -

  Fig. S3. Laser-scanning confocal microscopy was used to compare the distributions of (a) ASYM24 and (b) SC35 in MCF-7 cells treated with scramble siRNA duplexes in the experiment illustrated in Fig. 5Da.

• Supplemental Figure 4 -

  Fig. S4. MCF-7 cell extracts immunoprecipitated with anti-Sam68 and with non-immune serum, or the total cell extract, revealed with ASYM24. Migration profiles show that band b comigrates with Sam68 in the immunoprecipitate and in the cellular extract revealed with anti-Sam68 antibody. These results suggest that band b represents or at least contains Sam68.