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Fig. 2. IGF-1 exclusively signals to myotubes but not to reserve cells. (A) The ability of specific inhibitors of the p38 MAPK (SB203580; SB), p42 MAPK (PD098059; PD), and calcineurin (FK506; FK) to prevent the increase in cell fusion induced by IGF-1 was tested, as well as the ability of LiCl, inhibitor of GSK-3
, to mimic IGF-1-induced increase in fusion index. Above each column the significance of the sample versus the control (upper) and versus the IGF-treated sample (lower) is given; NS, non significant; **P<0.01 and ***P<0.001. (B) Calcineurin activity was measured in total cultures at day 3 of differentiation after 15 minutes treatment with IGF-1. (C) Activation of p38 MAPK, p42 MAPK and Akt after treatment with IGF-1 was investigated in total cultures at day 3 of differentiation by western blot analysis using antibodies specific for the phosphorylated forms of the molecules. (D) The phosphorylation of Akt, normalized by total Akt, and p42 MAPK was analyzed in myotubes and reserve cells separately, at day 3 of differentiation, after differential trypsinization, by western blot.
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