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Figure 4


Fig. 4. Treatment with exogenous IL-13 mimics the increase in fusion index and myotube size observed during IGF-1 induced human myotube hypertrophy. (A) The effects of exogenous treatment with IGF-1, IL-4 or IL-13 on the fusion index were determined. (B) The effects of exogenous treatment with IGF-1 and IL-13 at 5 ng/ml on the mean number of nuclei per myotube was measured. NS, non significant; *P<0.05; **P<0.01. (C) Immunostaining using desmin antibody was performed on cultures treated with IGF-1, IL-13 at 10 ng/ml or neutralizing antibody against IL-13. Nuclei were counterstained with Hoechst. Bar, 100 µm.





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