spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. IGF-1 stimulates protein metabolism via Akt (A) The ability of specific inhibitors of the p38 MAPK (SB=SB203580), p42 MAPK (PD=PD098059), the calcineurin (FK=FK506) and GSK-3beta (LiCl) to prevent the increase in myosin content induced by IGF-1 was tested. Above each column the significance of the sample versus the control (upper) and versus the IGF-treated sample (lower) is given; NS, non significant; *P<0.05; **P<0.01. (B) The phosphorylation of Foxo1, p70S6K, GSK-3beta and Akt was investigated by western blot analysis after treatment by IGF-1. (C) The effect of IGF-1 on the Foxo DNA binding activity of the cultures was investigated by EMSA. A competition with the unlabelled competitor oligo and a mutated probe were used to assess signal specificity. (D). The effect of IGF-1 on the expression of atrogin-1 was tested by northern blot analysis after a 3-hour treatment. Two mRNA forms of 2.4 kb and 6.5 kb were detected for atrogin-1, as described previously (Li et al., 2005).





Right arrow Return to article