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Figure 1


Fig. 1. (A,B) Cellular localization of the Clb2 mutants when expressed from the CLB2 endogenous promoter. WT, clb2{Delta}NLS and clb2L303 cells in which a GFP tag was introduced at the CLB2 locus were grown to mid-log-phase on YPD plates supplemented with adenine. All images were taken with the same optical settings and processed with identical parameters into Adobe PhotoShop. (A) Cellular distribution of Clb2NLS and Clb2L303A compared with the wild-type cyclin. (B) Representative images of the pattern of distribution of Clb2{Delta}NLS-GFP in late anaphase. Bar, 2µm.





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