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Fig. 6. The hyperpolarized phenotype of clb1 ${Delta}$ clb2^L303A cells is independent of CLN1,2. (A) clb1 ${Delta}$ and clb1 ${Delta}$ clb2^L303A cells expressing a CLN2-HA3 allele were released from an ${alpha}$ -factor arrest at 30°C and analyzed at the indicated times for the amount of Cln2-HA, Clb2 and Cdc28 by western blot analysis. (B) clb1 ${Delta}$ clb2^L303A and clb1 ${Delta}$ clb2^L303A cln1 ${Delta}$ cln2 ${Delta}$ cells were processed as described in the legend of Fig. 5C before staining for actin with Rhodamine-phalloidin. Bar, 2µm.

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