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Figure 5


Fig. 5. Expression of IL-1beta proteins in HEK 293 cells. (A) HEK 293 cells were transfected with empty vector (C), and constructs encoding Flag-labeled pro-IL-1beta (31), Flag-labeled 27-kDa IL-1beta (27) and Flag-labeled mature, 17-kDa IL-1beta (17). Cells were immunostained for IL-1beta (green) and cathepsin D (CD, red). Bar, 10 µm. (B) IL-1beta immunoblot of HEK 293 cell lysates transfected with empty vector (C), and constructs encoding Flag-labeled pro-IL-1beta (31), Flag-labeled 27-kDa IL-1beta (27) and - Flag-labeled mature, 17-kDa IL-1beta (17). Images and blots are representative of three independent experiments.





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