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Figure 2


Fig. 2. Mammalian Lis1 localizes to motile cilia in tracheae and oviduct. Immunofluorescence microscopy analysis using a goat polyclonal antibody (sc-2577) raised against human Lis1. (A) Tissue section of mouse trachea. Bar, 50 µm. Bar for insets, 10 µm. (B) Tissue section of mouse female reproductive organs. Main image (bar, 50 µm) and upper inset (bar, 10 µm) show motile cilia of the oviduct; lower inset (bar, 10 µm) shows a primary cilium in a follicular granulosa cell in the ovary. (C) Growth-arrested NIH3T3 fibroblast in culture. Main image (bar, 20 µm) and upper inset (bar, 8 µm) show localization of Lis1 along the cytoplasmic network of microtubules (open arrows); lower inset (bar, 20 µm) shows a primary cilium. Bold arrows indicate cilia. tb, acetylated {alpha}-tubulin.





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