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Figure 7


Fig. 7. CrLis1 sediments at ~3 S in sucrose density gradients. A Tergitol/ATP extract of Chlamydomonas flagella was fractionated in a 10-30% sucrose density gradient. Following electrophoresis, the fractions were probed with antibodies against ODA IC2 and LC1 to locate the ~20 S {alpha}beta HC complex and the 12 S {gamma} HC subunit, respectively. Under these conditions, CrLis1 sediments at ~3 S near the top of the gradient. Similar results were obtained when a 0.6 M NaCl axonemal extract was loaded on the gradient.





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