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Figure 2


Fig. 2. Stimulation of cardiomyogenesis of ES cells by H2O2. (A) ES-cell-derived embryoid bodies were treated on day 4 of differentiation with 500 nM H2O2 either in the presence and absence of the antioxidants vitamin E (100 µM) and NMPG (100 µM). H2O2 significantly increased the number of spontaneously contracting embryoid bodies on day 8 of cell culture, which was totally abolished in the presence of antioxidants. (B) Treatment of embryoid bodies with H2O2 significantly upregulated the cardiac-specific genes {alpha}-actin, ANP, beta-MHC, MLC2a and MLC2v. (C) Upregulation of {alpha}-actinin, MLC2a and cMLC1 protein by H2O2. The bar chart shows densitometric analyses of three independent experiments. *P<0.05, significantly different to the untreated control.





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