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Figure 7


Fig. 7. Analysis of proliferation markers in ES-cell-derived- and neonatal cardiac cells following treatment for 24 hours with 100 nM H2O2. Analysis of BrdU-positive cell nuclei in ES-cell-derived (A) and neonatal (B) cardiac cells. Nuclear/cytoplasmic (n/c) distribution of cyclin D1 in ES cell-derived (C) and neonatal (D) cardiac cells. Nuclear expression of Ki67 in ES cell-derived-(E) and neonatal (F) cardiac cells. Phosphorylation of Rb protein in ES-cell-derived (G) and neonatal (H) cardiac cells. Nuclear expression of p27kip1 in ES-cell-derived (I) and neonatal (J) cardiac cells. *P<0.05, significantly different to the untreated control.





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