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Figure 2


Fig. 2. Ultrastructure of DEPV-induced cardiomyocytes apoptosis. (A) Scanning electron micrographs of control cardiomyocytes (a) and those cultured under DEPV conditions (b-f). The DEPV-treated cardiomyocytes were characterized by detachment of the cell, which became spherical (b) as well as formation of many small blebs (arrowhead) on the cell surface (c-e) or apoptotic bodies (e,f; arrow). Bars, 10 µm. (B) Transmission electron micrographs of control cardiomyocytes (a) and those cultured under DEPV conditions (b-e). (a) The normal nucleus (NN), mitochondria (Mt), myofibrils (Mf) and cross striations (arrows) in a control cardiomyocyte. (b) The apoptotic nucleus (AN) with bounding condensed chromatin, shriveled cytoplasm, mitochondria (Mt), myofibrils (Mf) and a lot of vacuoles (arrowheads) in an apoptotic cardiomyocyte. (c) Apoptotic bodies (arrows) with condensed chromatin. (d) A phagocytosed apoptotic body (arrow). (e) Condensed chromatin in the round, oncotic nucleus (ON), disrupted organelles and droplets (L) in an oncotic cardiomyocyte. Bars, 2 µm.





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