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Figure 5


Fig. 5. Time-dependent action of DADLE on ERK phosphorylation, expression of survivin and Bcl-2, as well as Bax translocation from the cytosolic to the mitochondrial fraction in cardiomyocytes cultured under DEPV conditions. (A) Representative blots showing time course of p-ERK, ERK, survivin, Bcl-2 and the cytosolic and mitochondrial fractions of Bax in cardiomyocytes cultured under DEPV conditions with or without pretreatment with DADLE (A). (B-F) Quantification by densitometry of the bands of pERK (B), survivin (C), Bcl-2 (D), the mitochondrial fractions and the cytosolic fractions of Bax (E) (n=3 in each group). Values are the mean ± s.d. *P<0.05 between the indicated groups. DEPV, serum and glucose deprivation; DADLE, [D-Ala2, D-Leu5]-enkephalin acetate salt.





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