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Figure 6


Fig. 6. GFP-Sso1p accumulates in the ER in sporulating erv14{Delta} erv15{Delta} double mutants. The localization of GFP-Sso1p was examined in vegetative growth and sporulation in wild-type (AN120), erv14{Delta} (HI26), erv15{Delta} (YS229) and erv14{Delta} erv15{Delta} (YS232) strains. For analysis of vegetative cells, GFP-Sso1p was expressed under the control of TEF2 promoter from pRS424-TEF2pr-GFP-SSO1+3' and the ER was labeled with Kar2-RFP expressed from YEpGAPII-KmRFP. In sporulating cells, GFP-Sso1p and Sec61p-RFP were expressed from sporulation-specific promoters in plasmids pRS424-SPO20pr-GFP-SSO1+3' and pRS426-SPO20pr-SEC61-mRFP, respectively. In the bottom panel, localization of Sso1p to the prospore membrane in erv14{Delta} (HI26) cells was examined using the prospore membrane marker RFP-Spo2051-91. The corresponding DNA images are shown (DAPI). Bar, 1 µm.





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