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Figure 7


Fig. 7. Multiple proteins require ERV genes for localization to the prospore membrane during sporulation. Localization of Pma1p, Sso1p, Dtr1p and Sma2p during vegetative growth and sporulation in wild-type (AN120), erv14{Delta} (HI26), erv15{Delta} (YS229) and erv14{Delta} erv15{Delta} (YS232) strains was quantified using GFP fusions and ER markers as in Fig. 6. All the GFP fusions were expressed vegetatively under control of the TEF2 promoter and during sporulation under control of the SPO20 promoter except Dtr1p-GFP, which was expressed from its native promoter. The ER markers used were Kar2-RFP in vegetative cells and Sec61p-RFP in sporulating cells. For each strain at least 100 cells were examined and the percentage of cells showing localization of the GFP fusion to the plasma membrane during vegetative growth (open bars) or prospore membrane during sporulation (black bars) are shown. Asterisks in the Sma2-GFP graph indicate that the Sma2-GFP was mainly found diffusely throughout the cytoplasm in wild-type and erv15{Delta} cells during vegetative growth.





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