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Figure 4


Fig. 4. Apical localization does not correlate with DRM association. (A-C) Scanning confocal micrographs of MDCK cells stably transfected with cDNAs of indicated constructs. Cells were treated as in Fig. 2. Bars, 10 µm. (D-F) DRM association of NPP3-AAASLLAP (D), NPP1-{Delta}34 (E) and NPP1-AA (F). Cell homogenates were extracted and separated on sucrose gradients and the distribution of the proteins was analyzed as in Fig. 2. Typical immunoblots are shown. Bar chart values are means ± s.d. of at least three independent experiments.





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