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Figure 7


Fig. 7. Mutation of lysines 14-15 reduces affinity of NPP3 to Lubrol DRMs. (A) scanning confocal micrographs picture of MDCK cells stably expressing NPP3-KK/AA. Cells were labeled with specific monoclonal anti-NPP3 antibody followed by secondary FITC-conjugated antibody. Bar, 10 µm. (B) DRM association of NPP3-KK/AA. Cell homogenates were extracted and separated on sucrose gradients and the distribution of the proteins was analyzed as in Fig. 2. Typical immunoblots are shown. Bar chart values are means ± s.d. of two determinations.





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