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Figure 6


Fig. 6. Nocodazole does not affect VAMP4-EGFP recycling. After a 30-minute pretreatment with 33 µM nocodazole (a-d) or 0.1% DMSO (e-h), VAMP4-EGFP-expressing cells were incubated with rabbit anti-EGFP antibody for 30 minutes at 37°C in the continuous presence of nocodazole (a-d) or DMSO (e-h). Cells were then processed to reveal EGFP (green), anti-EGFP signals (red) and endogenous GS15 (blue). The merged images are also shown. Bar, 10 µm.





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