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Figure 1


Fig. 1. Detection of the interaction between Hus1 and Rad9 or Rad1. 1, L40 bearing Hus1 in pHybLex/Zeo vector and Rad9 in pYESTrp2 vector; 2, L40 bearing Hus1 in pHybLex/Zeo vector and Rad1 in pYESTrp2 vector; 3, L40 bearing Rad9 in pYESTrp2 vector and an empty pHybLex/Zeo vector; 4, L40 bearing Hus1 in pHybLex/Zeo vector and an empty pYESTrp2 vector; 5, L40 bearing Rad1 in pYESTrp2 vector and an empty pHybLex/Zeo vector. (A) Non-selective medium for detection of interaction; (B) The activation of the HIS promoter was tested on plates without histidine. (C) Activation of the lacZ promoter by assay of beta-galactosidase activity. Hus1 interacted either with Rad9 (B1,C1) or Rad1 (B2,C2).





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