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Fig. 2. Primary Timp3–/– MECs are more resistant to EGTA-induced cell detachment and have higher 
-catenin levels. (A) Representative images from at least three different experiments. An increased number of Timp3–/– epithelial islands (outlined with dotted circles) remain following EGTA treatment as assessed by Hoechst staining and fluorescence microscopy. Bars, 2 mm. (B) Representative western blots of whole-cell lysates from WT and Timp3–/– (t3–/–) MECs probed with the indicated antibodies. Quantification by densitometry of (C) E-cadherin signal and (D) -catenin signal (
, WT; 
, Timp3–/–; *P<0.05). (E) Western blot analyses of nuclear lysates of WT and Timp3–/– MECs using an anti--catenin antibody. (F) MEC total and nuclear lysates subjected to western blotting using an anti-dephosphorylated -catenin (de-P--cat). Panels B-F represent one of three pools of primary mammary epithelial cultures. Each MEC pool was harvested from all ten mammary glands of five WT and five Timp3–/– female mice. Amido Black and silver staining were used to assess protein loading. Student's t-test was performed to assess statistical significance between WT and Timp3–/–. Bar graphs were expressed as the mean ± s.e.m.
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