|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Metalloproteinase inhibitors decrease 
-catenin signaling in Timp3–/– cells. (A) Western blots of whole-cell lysates from WT and Timp3–/– MECs revealed that metalloproteinase inhibition with recombinant TIMP3 (25 µM), GM6001 (25 µM) and PD166793 (50 µM) for 24 hours returned dephosphorylated -catenin to levels comparable to the WT. (B) The reduction in Ccnd1 gene expression in Timp3–/– MECs was reversed by 24-hour treatment with recombinant TIMP3 (10 µM) (*P<0.001), GM6001 (10 µM) (**P<0.01) and PD166793 (25 µM) (***P<0.05) when compared with the untreated control. (C) The increase in Mmp7 gene expression in Timp3–/– MECs was significantly mitigated by treatment with recombinant TIMP3 (10 µM) (*P<0.001), GM6001 (10 µM) (*P<0.001) and PD166793 (25 µM) (**P<0.05) for 24 hours. ANOVA was performed to assess the statistical significance between WT and Timp3–/– cells. Values are expressed as mean ± s.e.m. of three independent experiments.
|
