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Figure 2


Fig. 2. C1A9 and Me(3)K9H3 staining in nuclei of cells from a tract of a male gut undergoing developmental reversal of heterochromatinisation. (A) Dissected gut of a male mealybug. (B) DAPI staining of nuclei, showing that the chromocenters of nuclei undergoing reversal of heterochromatinisation are decondensed and have lost their focal morphological appearance. (C,D) C1A9 staining of these nuclei is grainy and dispersed over the nucleus (C), whereas Me(3)K9H3 is still concentrated over the decondensed chromocenter (D). (E) Merged image. Bars, 10 µm.





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