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Figure 5


Fig. 5. pchet2 dsRNA has an effect after 2 hours and mitotic chromosomes are particularly sensitive to the treatment. (A) DAPI staining of a 128-256-nuclei male embryo treated for 2 hours showing a decondensing chromocenter and detectable C1A9-staining that is becoming dispersed and more grainy in appearance (A'). Mitotic chromosomes show a marked reduction in C1A9 staining. (B,B') Both mitotic chromosomes and an interphase nucleus are stained with DAPI and C1A9, respectively. In B' the mitotic chromosomes are not stained with C1A9, whereas the interphase nuclei still possess a C1A9-positive chromocenter (arrow in B'). After 4 hours the chromocenters have been almost completely disassembled, with only remnants remaining (arrows in C and D). The remnants did not stain with C1A9 (C',D') or Me(3)K9H3 (C"), or with Me(3)K20H4 (D"). Bars, 10 µm.





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