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Figure 6


Fig. 6. pchet2-dsRNA-treated embryos of both sexes exhibit defects in chromosome condensation and integrity as well as segregation defects. (A-E) DAPI-stained nuclei from pchet2-dsRNA-treated 128-256-stage embryos often possess highly elongated chromosomes that result from incomplete condensation. Mitotic figures also contain `dot' chromosomes (arrows in B and C). pchet2-RNA-treated embryos exhibit segregation defects, showing chromosomes that are displaced from the metaphase plate (arrows in D and E). For comparison, a normal metaphase from mocktreated embryos is shown in F. Bar, 10 µm.





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