JCS03407 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Trailer Hitch colocalizes with Rtnl1GFP on the fusome and in nurse cells. (A) Like Orb and Me31b, Trailer Hitch (Tral) colocalizes with Rtnl1GFP on the central portion of the fusome in the germarium. Shown is a z-stack of confocal sections covering the whole germarium, Rtnl1GFP is in green in A and as a single channel in A’, and Trailer Hitch is in red in A and as a single channel in A’’. (B-D) Comparison of Trailer Hitch localization in nurse cells with Rtnl1GFP (B), Sec61αGFP (C) and PDI-GFP (D). B Foci of Trailer Hitch colocalize with foci of stronger Rtnl1GFP labeling in the nurse cells. C, D Foci of Trailer Hitch localize to regions of Sec61αGFP and PDI-GFP labeling in the nurse cells, though both ER markers are not more enriched in these foci in contrast to Rtnl1GFP. Rtnl1GFP, Sec61αGFP and PDI-GFP are in green in B-D, and as single channels in B’-D’, Trailer Hitch is in red in B-D and as a single channel in B’’-D’’. Bars, 10 μm.

• Movie 1 -

  Movie 1. The movie shows a 3D-reconstructed z-stack of region 2 of a germarium with Rtnl1-GFP in green and antibody staining for Hts in red. Note that Rtnl1-GFP appears to be throughout but also surrounding the structure marked by Hts