spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Comparison of Rtnl1-GFP, PDI-GFP and Sec61{alpha}-GFP in various embryonic tissues during development. (A-M') The labeling of Rtnl1, PDI and Sec61{alpha} by exon-trapping with GFP shows the endogenous expression patterns of all three proteins. All highlight similar ER structures in the early syncytial embryo before cellularization (A-C) and later within the embryonic epidermis (K-M, shown is the epidermis-amnioserosa interface), although levels differ in stage 14 embryos. Note the striking differences in expression within the CNS (D-F) where Rtnl1-GFP (D) is strongly concentrated in the axonal tracts but both PDI-GFP (E) and Sec61{alpha}-GFP (F) are completely absent. Rtnl1-GFP is enriched in the SR (a specialization of smooth ER) of embryonic muscles (G), whereas PDI-GFP and Sec61{alpha}-GFP strongly mark the perinuclear rough ER in muscles (H and I, respectively). G'-I' show higher magnifications of the areas indicated by red boxes in G-I. Arrows in G' and H' point to the SR, arrowheads in H' and I' point to the nuclear envelope. Note that the bright staining in G'-I' is the labeling within the embryonic epidermis and not the muscle. Bars, 10 µm.





Right arrow Return to article