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Figure 7


Fig. 7. Orb colocalizes with Rtnl1 on both the fusome-ER and the oocyte-ER. (A-B") The RNA-binding protein Orb (red in A and B and as a single channel in A" and B") colocalizes with Rtnl1-GFP (green in A and B and as a single channel in A' and B') on the central portion of the fusome. This is visible in both projections of confocal z-stacks (A-A") and also single confocal sections (B-B"). Arrows point to the central portion of the fusome in the germarium and the posterior crescent of Orb and Rtnl1-GFP in a budding egg chamber. (C-D") Orb and Rtnl1-GFP also colocalize in punctate structures in oocytes of stage 6 (C-C") and stage 8 (D-D") egg chambers. Rtnl1-GFP is shown in green in C and D and as a single channel in C' and D', Orb is in red in C and D and as a single channel in C" and D". Arrows in C',C",D' and D" point to colocalizing foci. Bars, 10 µm.





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